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pbabe hygro htert plasmid  (Addgene inc)


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    Structured Review

    Addgene inc pbabe hygro htert plasmid
    Pbabe Hygro Htert Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 103 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbabe-hygro/pBABE-hygro-hTERT+(Plasmid+%231773)/pmc12961426-53-23-25
    Average 93 stars, based on 103 article reviews
    pbabe hygro htert plasmid - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Vimentin intermediate filaments and filamentous actin form unexpected interpenetrating networks that redefine the cell cortex
    Article Snippet: The PCR product is purified from an agarose gel and inserted into pBABE-hygro (pBABE-hygro is a gift from Hartmut Land and Jay P. Morgenstern, Imperial Cancer Research Fund, Lincoln's Inn Fields, London ( ) [Addgene plasmid no. 1765; http://n2t.net/addgene:1765 ; RRID Addgene_1765]) cut with BamHI and EcoRI using In-Fusion (Clontech).

    Article Title: Mouse Genetics Suggests Cell-Context Dependency for Myc-Regulated Metabolic Enzymes during Tumorigenesis
    Article Snippet: For retroviruses the following plasmids from Addgene were used: pBABE-HrasG12V-puro, pBABE-HrasG12V-hygro, MSCV- Myc -IRES-GFP, pWZL-Myc-blasticidine, pBABE-puro, pBABE-hygro together with pCL-Eco (encoding gag, pol and ecotropic envelope).

    Article Title: VPS37A directs ESCRT recruitment for phagophore closure
    Article Snippet: The following plasmids were obtained from Hartmut Land (University of Rochester Medical Center, Rochester, NY), Jay Morgenstern (Imperial Cancer Research Fund, London, UK), Bob Weinberg (Whitehead Institute for Biomedical Research, Cambridge, MA), Feng Zhang (Massachusetts Institute of Technology, Cambridge, MA), Brett Stringer (QIMR Berghofer Medical Research Institute, Brisbane, Australia), Christopher Vakoc (Cold Spring Harbor Laboratory, Cold Spring Harbor, NY), Noboru Mizushima (The University of Tokyo, Tokyo, Japan), Wesley Sundquist (University of Utah School of Medicine, Salt Lake City, UT), Konrad Buessow (Max Planck Institute for Molecular Genetics, Berlin, Germany), and Hemmo Meyer (University of Duisburg-Essen, Essen, Germany) through Addgene: pBabe-Hygro (#1765; ), lentiCas9-Blast (#52962; ), lentiCRISPRv2 (puro; #98290; Brett Stringer Lab), LRG (Lenti_sgRNA_EFS_GFP, #65656; ), pMXs-IP-EGFP-mATG5 (#38196; ), pMXs-IP-EGFP-ULK1 (#38193; ), pMXs-IP-EGFP-LC3 (#38195; ), pEGFP-VPS4-E228Q (#80351; ), pQTEV-VPS28 (#34803; ), and pmCherry-GAL3 (#85662; ).

    Article Title: Mouse genetics suggests cell-context dependency for Myc-regulated metabolic enzymes during tumorigenesis.
    Article Snippet: For retroviruses the following plasmids from Addgene were used: pBABEHrasG12V-puro, pBABE-HrasG12V-hygro, MSCV-Myc-IRESGFP, pWZL-Myc-blasticidine, pBABE-puro, pBABE-hygro together with pCL-Eco (encoding gag, pol and ecotropic envelope).

    Article Title: Progerin reduces LAP2α-telomere association in Hutchinson-Gilford progeria
    Article Snippet: LAP2α was amplified from pTD15 (gift from Dr Roland Foisner, Vienna). cDNAs were cloned into retroviral vector pBABE-hygro (Addgene, Cambridge, MA) or doxycyclin-inducible lentiviral vector pTRIPZ (Open Biosystem, Singapore).

    Article Title: A Role for p53 in the Adaptation to Glutamine Starvation through the Expression of SLC1A3
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Software and Algorithms GraphPad Prism 7 GraphPad software N/A FlowJo software v.10.3 FlowJo N/A CRISPR Design Tool Feng Zhang Lab (MIT) http://tools.genome-engineering.org CRISPOR Design Tool Tefor Infrastructure http://crispor.tefor.net/crispor.py IPA Qiagen https://www.qiagenbioinformatics.com/ products/ingenuity-pathway-analysis/ TopHat2 Kim et al., 2013 https://ccb.jhu.edu/software/tophat/ index.shtml HTSeq v.0.10.0 Simon Anders (EMBL Heidelberg) https://htseq.readthedocs.io/en/ release_0.10.0/ DESeq2 Love et al., 2014 https://bioconductor.org/packages/ release/bioc/html/DESeq2.html Cufflinks Trapnell et al., 2012 http://cole-trapnell-lab.github.io/ cufflinks/cuffdiff/ TraceFinder Version 4.1 Thermo Fisher Scientific OPTON-30626 Image Studio Lite Version 5.2.5 LI-COR https://www.licor.com/bio/products/ software/image_studio/

    Article Title: CD44 correlates with longevity and enhances basal ATF6 activity and ER stress resistance.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER pBabe-hygro Morgenstern and Land68 Addgene (#1765) pBabe-hygro-hTert Counter et al.69 Addgene (#1773) pBabe-hygro-mouse-CD44 This paper N/A pBabe-hygro-NMR-CD44 This paper N/A pBabe-hygro-mouse-LDLR This paper N/A pBabe-hygro-human-ATF6 (wild-type) This paper N/A pBabe-hygro-human-ATF6 (Y392C) This paper N/A pBabe-hygro-human-ATF6 (Y392C) This paper N/A pBabe-puro-human-CD44 Godar et al.70 Addgene (#19127) pBabe-puro-CD44-ectodomain-KDEL This paper N/A pLKO.1 Stewart et al.71 Addgene (#8453) pLKO.1-shLuc This paper N/A pLKO.1-shCD44-1 This paper N/A pLKO.1-shCD44-2 This paper N/A pDonor-tBFP-NLS-Neo Katoh et al.72 80767 peSpCas9(1.1)-23sgRNA Katoh et al.72 80768 Software and algorithms GraphPad Prism7 https://www.graphpad.com/ N/A ProteinPilot https://sciex.com/products/software/ proteinpilot-software 5.0.1 Progenesis QI https://www.nonlinear.com/progenesis/qi/ 4.2 FlowJo https://www.flowjo.com/ 10.7.1 Enrichr https://maayanlab.cloud/Enrichr/ N/A DESeq2 https://bioconductor.org 1.12.0 EdgeR https://bioconductor.org 3.32.0 limma https://bioconductor.org 3.46.0 genefilter https://bioconductor.org 1.72.0 TCC https://bioconductor.org 1.30.0 RSEM https://github.com/deweylab/RSEM 1.3.0 Trimmomatic http://www.usadellab.org/cms/? page = trimmomatic 0.38

    Article Title: CUX1 Enhances Pancreatic Cancer Formation by Synergizing with KRAS and Inducing MEK/ERK-Dependent Proliferation
    Article Snippet: The oncogenic KrasG12D was cloned from the pBabe-puro vector, a kind gift from Channing Der (Addgene plasmid # 58902, Watertown, MA, USA), into the pBabe-hygro, a gift from Hartmut Land, Jay Morgenstern and Bob Weinberg (Addgene plasmid # 1765, Watertown, MA, USA) using the BamHI and SalI restriction sites.

    Purification:

    Article Title: Vimentin intermediate filaments and filamentous actin form unexpected interpenetrating networks that redefine the cell cortex
    Article Snippet: The PCR product is purified from an agarose gel and inserted into pBABE-hygro (pBABE-hygro is a gift from Hartmut Land and Jay P. Morgenstern, Imperial Cancer Research Fund, Lincoln's Inn Fields, London ( ) [Addgene plasmid no. 1765; http://n2t.net/addgene:1765 ; RRID Addgene_1765]) cut with BamHI and EcoRI using In-Fusion (Clontech).

    Article Title: Mouse Genetics Suggests Cell-Context Dependency for Myc-Regulated Metabolic Enzymes during Tumorigenesis
    Article Snippet: For retroviruses the following plasmids from Addgene were used: pBABE-HrasG12V-puro, pBABE-HrasG12V-hygro, MSCV- Myc -IRES-GFP, pWZL-Myc-blasticidine, pBABE-puro, pBABE-hygro together with pCL-Eco (encoding gag, pol and ecotropic envelope).

    Article Title: VPS37A directs ESCRT recruitment for phagophore closure
    Article Snippet: The following plasmids were obtained from Hartmut Land (University of Rochester Medical Center, Rochester, NY), Jay Morgenstern (Imperial Cancer Research Fund, London, UK), Bob Weinberg (Whitehead Institute for Biomedical Research, Cambridge, MA), Feng Zhang (Massachusetts Institute of Technology, Cambridge, MA), Brett Stringer (QIMR Berghofer Medical Research Institute, Brisbane, Australia), Christopher Vakoc (Cold Spring Harbor Laboratory, Cold Spring Harbor, NY), Noboru Mizushima (The University of Tokyo, Tokyo, Japan), Wesley Sundquist (University of Utah School of Medicine, Salt Lake City, UT), Konrad Buessow (Max Planck Institute for Molecular Genetics, Berlin, Germany), and Hemmo Meyer (University of Duisburg-Essen, Essen, Germany) through Addgene: pBabe-Hygro (#1765; ), lentiCas9-Blast (#52962; ), lentiCRISPRv2 (puro; #98290; Brett Stringer Lab), LRG (Lenti_sgRNA_EFS_GFP, #65656; ), pMXs-IP-EGFP-mATG5 (#38196; ), pMXs-IP-EGFP-ULK1 (#38193; ), pMXs-IP-EGFP-LC3 (#38195; ), pEGFP-VPS4-E228Q (#80351; ), pQTEV-VPS28 (#34803; ), and pmCherry-GAL3 (#85662; ).

    Article Title: Mouse genetics suggests cell-context dependency for Myc-regulated metabolic enzymes during tumorigenesis.
    Article Snippet: For retroviruses the following plasmids from Addgene were used: pBABEHrasG12V-puro, pBABE-HrasG12V-hygro, MSCV-Myc-IRESGFP, pWZL-Myc-blasticidine, pBABE-puro, pBABE-hygro together with pCL-Eco (encoding gag, pol and ecotropic envelope).

    Article Title: Progerin reduces LAP2α-telomere association in Hutchinson-Gilford progeria
    Article Snippet: LAP2α was amplified from pTD15 (gift from Dr Roland Foisner, Vienna). cDNAs were cloned into retroviral vector pBABE-hygro (Addgene, Cambridge, MA) or doxycyclin-inducible lentiviral vector pTRIPZ (Open Biosystem, Singapore).

    Article Title: A Role for p53 in the Adaptation to Glutamine Starvation through the Expression of SLC1A3
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Software and Algorithms GraphPad Prism 7 GraphPad software N/A FlowJo software v.10.3 FlowJo N/A CRISPR Design Tool Feng Zhang Lab (MIT) http://tools.genome-engineering.org CRISPOR Design Tool Tefor Infrastructure http://crispor.tefor.net/crispor.py IPA Qiagen https://www.qiagenbioinformatics.com/ products/ingenuity-pathway-analysis/ TopHat2 Kim et al., 2013 https://ccb.jhu.edu/software/tophat/ index.shtml HTSeq v.0.10.0 Simon Anders (EMBL Heidelberg) https://htseq.readthedocs.io/en/ release_0.10.0/ DESeq2 Love et al., 2014 https://bioconductor.org/packages/ release/bioc/html/DESeq2.html Cufflinks Trapnell et al., 2012 http://cole-trapnell-lab.github.io/ cufflinks/cuffdiff/ TraceFinder Version 4.1 Thermo Fisher Scientific OPTON-30626 Image Studio Lite Version 5.2.5 LI-COR https://www.licor.com/bio/products/ software/image_studio/

    Article Title: CD44 correlates with longevity and enhances basal ATF6 activity and ER stress resistance.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER pBabe-hygro Morgenstern and Land68 Addgene (#1765) pBabe-hygro-hTert Counter et al.69 Addgene (#1773) pBabe-hygro-mouse-CD44 This paper N/A pBabe-hygro-NMR-CD44 This paper N/A pBabe-hygro-mouse-LDLR This paper N/A pBabe-hygro-human-ATF6 (wild-type) This paper N/A pBabe-hygro-human-ATF6 (Y392C) This paper N/A pBabe-hygro-human-ATF6 (Y392C) This paper N/A pBabe-puro-human-CD44 Godar et al.70 Addgene (#19127) pBabe-puro-CD44-ectodomain-KDEL This paper N/A pLKO.1 Stewart et al.71 Addgene (#8453) pLKO.1-shLuc This paper N/A pLKO.1-shCD44-1 This paper N/A pLKO.1-shCD44-2 This paper N/A pDonor-tBFP-NLS-Neo Katoh et al.72 80767 peSpCas9(1.1)-23sgRNA Katoh et al.72 80768 Software and algorithms GraphPad Prism7 https://www.graphpad.com/ N/A ProteinPilot https://sciex.com/products/software/ proteinpilot-software 5.0.1 Progenesis QI https://www.nonlinear.com/progenesis/qi/ 4.2 FlowJo https://www.flowjo.com/ 10.7.1 Enrichr https://maayanlab.cloud/Enrichr/ N/A DESeq2 https://bioconductor.org 1.12.0 EdgeR https://bioconductor.org 3.32.0 limma https://bioconductor.org 3.46.0 genefilter https://bioconductor.org 1.72.0 TCC https://bioconductor.org 1.30.0 RSEM https://github.com/deweylab/RSEM 1.3.0 Trimmomatic http://www.usadellab.org/cms/? page = trimmomatic 0.38

    Article Title: CUX1 Enhances Pancreatic Cancer Formation by Synergizing with KRAS and Inducing MEK/ERK-Dependent Proliferation
    Article Snippet: The oncogenic KrasG12D was cloned from the pBabe-puro vector, a kind gift from Channing Der (Addgene plasmid # 58902, Watertown, MA, USA), into the pBabe-hygro, a gift from Hartmut Land, Jay Morgenstern and Bob Weinberg (Addgene plasmid # 1765, Watertown, MA, USA) using the BamHI and SalI restriction sites.

    Agarose Gel Electrophoresis:

    Article Title: Vimentin intermediate filaments and filamentous actin form unexpected interpenetrating networks that redefine the cell cortex
    Article Snippet: The PCR product is purified from an agarose gel and inserted into pBABE-hygro (pBABE-hygro is a gift from Hartmut Land and Jay P. Morgenstern, Imperial Cancer Research Fund, Lincoln's Inn Fields, London ( ) [Addgene plasmid no. 1765; http://n2t.net/addgene:1765 ; RRID Addgene_1765]) cut with BamHI and EcoRI using In-Fusion (Clontech).

    Article Title: Mouse Genetics Suggests Cell-Context Dependency for Myc-Regulated Metabolic Enzymes during Tumorigenesis
    Article Snippet: For retroviruses the following plasmids from Addgene were used: pBABE-HrasG12V-puro, pBABE-HrasG12V-hygro, MSCV- Myc -IRES-GFP, pWZL-Myc-blasticidine, pBABE-puro, pBABE-hygro together with pCL-Eco (encoding gag, pol and ecotropic envelope).

    Article Title: VPS37A directs ESCRT recruitment for phagophore closure
    Article Snippet: The following plasmids were obtained from Hartmut Land (University of Rochester Medical Center, Rochester, NY), Jay Morgenstern (Imperial Cancer Research Fund, London, UK), Bob Weinberg (Whitehead Institute for Biomedical Research, Cambridge, MA), Feng Zhang (Massachusetts Institute of Technology, Cambridge, MA), Brett Stringer (QIMR Berghofer Medical Research Institute, Brisbane, Australia), Christopher Vakoc (Cold Spring Harbor Laboratory, Cold Spring Harbor, NY), Noboru Mizushima (The University of Tokyo, Tokyo, Japan), Wesley Sundquist (University of Utah School of Medicine, Salt Lake City, UT), Konrad Buessow (Max Planck Institute for Molecular Genetics, Berlin, Germany), and Hemmo Meyer (University of Duisburg-Essen, Essen, Germany) through Addgene: pBabe-Hygro (#1765; ), lentiCas9-Blast (#52962; ), lentiCRISPRv2 (puro; #98290; Brett Stringer Lab), LRG (Lenti_sgRNA_EFS_GFP, #65656; ), pMXs-IP-EGFP-mATG5 (#38196; ), pMXs-IP-EGFP-ULK1 (#38193; ), pMXs-IP-EGFP-LC3 (#38195; ), pEGFP-VPS4-E228Q (#80351; ), pQTEV-VPS28 (#34803; ), and pmCherry-GAL3 (#85662; ).

    Article Title: Mouse genetics suggests cell-context dependency for Myc-regulated metabolic enzymes during tumorigenesis.
    Article Snippet: For retroviruses the following plasmids from Addgene were used: pBABEHrasG12V-puro, pBABE-HrasG12V-hygro, MSCV-Myc-IRESGFP, pWZL-Myc-blasticidine, pBABE-puro, pBABE-hygro together with pCL-Eco (encoding gag, pol and ecotropic envelope).

    Article Title: Progerin reduces LAP2α-telomere association in Hutchinson-Gilford progeria
    Article Snippet: LAP2α was amplified from pTD15 (gift from Dr Roland Foisner, Vienna). cDNAs were cloned into retroviral vector pBABE-hygro (Addgene, Cambridge, MA) or doxycyclin-inducible lentiviral vector pTRIPZ (Open Biosystem, Singapore).

    Article Title: A Role for p53 in the Adaptation to Glutamine Starvation through the Expression of SLC1A3
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Software and Algorithms GraphPad Prism 7 GraphPad software N/A FlowJo software v.10.3 FlowJo N/A CRISPR Design Tool Feng Zhang Lab (MIT) http://tools.genome-engineering.org CRISPOR Design Tool Tefor Infrastructure http://crispor.tefor.net/crispor.py IPA Qiagen https://www.qiagenbioinformatics.com/ products/ingenuity-pathway-analysis/ TopHat2 Kim et al., 2013 https://ccb.jhu.edu/software/tophat/ index.shtml HTSeq v.0.10.0 Simon Anders (EMBL Heidelberg) https://htseq.readthedocs.io/en/ release_0.10.0/ DESeq2 Love et al., 2014 https://bioconductor.org/packages/ release/bioc/html/DESeq2.html Cufflinks Trapnell et al., 2012 http://cole-trapnell-lab.github.io/ cufflinks/cuffdiff/ TraceFinder Version 4.1 Thermo Fisher Scientific OPTON-30626 Image Studio Lite Version 5.2.5 LI-COR https://www.licor.com/bio/products/ software/image_studio/

    Article Title: CD44 correlates with longevity and enhances basal ATF6 activity and ER stress resistance.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER pBabe-hygro Morgenstern and Land68 Addgene (#1765) pBabe-hygro-hTert Counter et al.69 Addgene (#1773) pBabe-hygro-mouse-CD44 This paper N/A pBabe-hygro-NMR-CD44 This paper N/A pBabe-hygro-mouse-LDLR This paper N/A pBabe-hygro-human-ATF6 (wild-type) This paper N/A pBabe-hygro-human-ATF6 (Y392C) This paper N/A pBabe-hygro-human-ATF6 (Y392C) This paper N/A pBabe-puro-human-CD44 Godar et al.70 Addgene (#19127) pBabe-puro-CD44-ectodomain-KDEL This paper N/A pLKO.1 Stewart et al.71 Addgene (#8453) pLKO.1-shLuc This paper N/A pLKO.1-shCD44-1 This paper N/A pLKO.1-shCD44-2 This paper N/A pDonor-tBFP-NLS-Neo Katoh et al.72 80767 peSpCas9(1.1)-23sgRNA Katoh et al.72 80768 Software and algorithms GraphPad Prism7 https://www.graphpad.com/ N/A ProteinPilot https://sciex.com/products/software/ proteinpilot-software 5.0.1 Progenesis QI https://www.nonlinear.com/progenesis/qi/ 4.2 FlowJo https://www.flowjo.com/ 10.7.1 Enrichr https://maayanlab.cloud/Enrichr/ N/A DESeq2 https://bioconductor.org 1.12.0 EdgeR https://bioconductor.org 3.32.0 limma https://bioconductor.org 3.46.0 genefilter https://bioconductor.org 1.72.0 TCC https://bioconductor.org 1.30.0 RSEM https://github.com/deweylab/RSEM 1.3.0 Trimmomatic http://www.usadellab.org/cms/? page = trimmomatic 0.38

    Article Title: CUX1 Enhances Pancreatic Cancer Formation by Synergizing with KRAS and Inducing MEK/ERK-Dependent Proliferation
    Article Snippet: The oncogenic KrasG12D was cloned from the pBabe-puro vector, a kind gift from Channing Der (Addgene plasmid # 58902, Watertown, MA, USA), into the pBabe-hygro, a gift from Hartmut Land, Jay Morgenstern and Bob Weinberg (Addgene plasmid # 1765, Watertown, MA, USA) using the BamHI and SalI restriction sites.

    Plasmid Preparation:

    Article Title: Vimentin intermediate filaments and filamentous actin form unexpected interpenetrating networks that redefine the cell cortex
    Article Snippet: The PCR product is purified from an agarose gel and inserted into pBABE-hygro (pBABE-hygro is a gift from Hartmut Land and Jay P. Morgenstern, Imperial Cancer Research Fund, Lincoln's Inn Fields, London ( ) [Addgene plasmid no. 1765; http://n2t.net/addgene:1765 ; RRID Addgene_1765]) cut with BamHI and EcoRI using In-Fusion (Clontech).

    Article Title: Mouse Genetics Suggests Cell-Context Dependency for Myc-Regulated Metabolic Enzymes during Tumorigenesis
    Article Snippet: For retroviruses the following plasmids from Addgene were used: pBABE-HrasG12V-puro, pBABE-HrasG12V-hygro, MSCV- Myc -IRES-GFP, pWZL-Myc-blasticidine, pBABE-puro, pBABE-hygro together with pCL-Eco (encoding gag, pol and ecotropic envelope).

    Article Title: VPS37A directs ESCRT recruitment for phagophore closure
    Article Snippet: The following plasmids were obtained from Hartmut Land (University of Rochester Medical Center, Rochester, NY), Jay Morgenstern (Imperial Cancer Research Fund, London, UK), Bob Weinberg (Whitehead Institute for Biomedical Research, Cambridge, MA), Feng Zhang (Massachusetts Institute of Technology, Cambridge, MA), Brett Stringer (QIMR Berghofer Medical Research Institute, Brisbane, Australia), Christopher Vakoc (Cold Spring Harbor Laboratory, Cold Spring Harbor, NY), Noboru Mizushima (The University of Tokyo, Tokyo, Japan), Wesley Sundquist (University of Utah School of Medicine, Salt Lake City, UT), Konrad Buessow (Max Planck Institute for Molecular Genetics, Berlin, Germany), and Hemmo Meyer (University of Duisburg-Essen, Essen, Germany) through Addgene: pBabe-Hygro (#1765; ), lentiCas9-Blast (#52962; ), lentiCRISPRv2 (puro; #98290; Brett Stringer Lab), LRG (Lenti_sgRNA_EFS_GFP, #65656; ), pMXs-IP-EGFP-mATG5 (#38196; ), pMXs-IP-EGFP-ULK1 (#38193; ), pMXs-IP-EGFP-LC3 (#38195; ), pEGFP-VPS4-E228Q (#80351; ), pQTEV-VPS28 (#34803; ), and pmCherry-GAL3 (#85662; ).

    Article Title: Mouse genetics suggests cell-context dependency for Myc-regulated metabolic enzymes during tumorigenesis.
    Article Snippet: For retroviruses the following plasmids from Addgene were used: pBABEHrasG12V-puro, pBABE-HrasG12V-hygro, MSCV-Myc-IRESGFP, pWZL-Myc-blasticidine, pBABE-puro, pBABE-hygro together with pCL-Eco (encoding gag, pol and ecotropic envelope).

    Article Title: Progerin reduces LAP2α-telomere association in Hutchinson-Gilford progeria
    Article Snippet: LAP2α was amplified from pTD15 (gift from Dr Roland Foisner, Vienna). cDNAs were cloned into retroviral vector pBABE-hygro (Addgene, Cambridge, MA) or doxycyclin-inducible lentiviral vector pTRIPZ (Open Biosystem, Singapore).

    Article Title: A Role for p53 in the Adaptation to Glutamine Starvation through the Expression of SLC1A3
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Software and Algorithms GraphPad Prism 7 GraphPad software N/A FlowJo software v.10.3 FlowJo N/A CRISPR Design Tool Feng Zhang Lab (MIT) http://tools.genome-engineering.org CRISPOR Design Tool Tefor Infrastructure http://crispor.tefor.net/crispor.py IPA Qiagen https://www.qiagenbioinformatics.com/ products/ingenuity-pathway-analysis/ TopHat2 Kim et al., 2013 https://ccb.jhu.edu/software/tophat/ index.shtml HTSeq v.0.10.0 Simon Anders (EMBL Heidelberg) https://htseq.readthedocs.io/en/ release_0.10.0/ DESeq2 Love et al., 2014 https://bioconductor.org/packages/ release/bioc/html/DESeq2.html Cufflinks Trapnell et al., 2012 http://cole-trapnell-lab.github.io/ cufflinks/cuffdiff/ TraceFinder Version 4.1 Thermo Fisher Scientific OPTON-30626 Image Studio Lite Version 5.2.5 LI-COR https://www.licor.com/bio/products/ software/image_studio/

    Article Title: CD44 correlates with longevity and enhances basal ATF6 activity and ER stress resistance.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER pBabe-hygro Morgenstern and Land68 Addgene (#1765) pBabe-hygro-hTert Counter et al.69 Addgene (#1773) pBabe-hygro-mouse-CD44 This paper N/A pBabe-hygro-NMR-CD44 This paper N/A pBabe-hygro-mouse-LDLR This paper N/A pBabe-hygro-human-ATF6 (wild-type) This paper N/A pBabe-hygro-human-ATF6 (Y392C) This paper N/A pBabe-hygro-human-ATF6 (Y392C) This paper N/A pBabe-puro-human-CD44 Godar et al.70 Addgene (#19127) pBabe-puro-CD44-ectodomain-KDEL This paper N/A pLKO.1 Stewart et al.71 Addgene (#8453) pLKO.1-shLuc This paper N/A pLKO.1-shCD44-1 This paper N/A pLKO.1-shCD44-2 This paper N/A pDonor-tBFP-NLS-Neo Katoh et al.72 80767 peSpCas9(1.1)-23sgRNA Katoh et al.72 80768 Software and algorithms GraphPad Prism7 https://www.graphpad.com/ N/A ProteinPilot https://sciex.com/products/software/ proteinpilot-software 5.0.1 Progenesis QI https://www.nonlinear.com/progenesis/qi/ 4.2 FlowJo https://www.flowjo.com/ 10.7.1 Enrichr https://maayanlab.cloud/Enrichr/ N/A DESeq2 https://bioconductor.org 1.12.0 EdgeR https://bioconductor.org 3.32.0 limma https://bioconductor.org 3.46.0 genefilter https://bioconductor.org 1.72.0 TCC https://bioconductor.org 1.30.0 RSEM https://github.com/deweylab/RSEM 1.3.0 Trimmomatic http://www.usadellab.org/cms/? page = trimmomatic 0.38

    Article Title: CUX1 Enhances Pancreatic Cancer Formation by Synergizing with KRAS and Inducing MEK/ERK-Dependent Proliferation
    Article Snippet: The oncogenic KrasG12D was cloned from the pBabe-puro vector, a kind gift from Channing Der (Addgene plasmid # 58902, Watertown, MA, USA), into the pBabe-hygro, a gift from Hartmut Land, Jay Morgenstern and Bob Weinberg (Addgene plasmid # 1765, Watertown, MA, USA) using the BamHI and SalI restriction sites.

    Clone Assay:

    Article Title: Vimentin intermediate filaments and filamentous actin form unexpected interpenetrating networks that redefine the cell cortex
    Article Snippet: The PCR product is purified from an agarose gel and inserted into pBABE-hygro (pBABE-hygro is a gift from Hartmut Land and Jay P. Morgenstern, Imperial Cancer Research Fund, Lincoln's Inn Fields, London ( ) [Addgene plasmid no. 1765; http://n2t.net/addgene:1765 ; RRID Addgene_1765]) cut with BamHI and EcoRI using In-Fusion (Clontech).

    Article Title: Mouse Genetics Suggests Cell-Context Dependency for Myc-Regulated Metabolic Enzymes during Tumorigenesis
    Article Snippet: For retroviruses the following plasmids from Addgene were used: pBABE-HrasG12V-puro, pBABE-HrasG12V-hygro, MSCV- Myc -IRES-GFP, pWZL-Myc-blasticidine, pBABE-puro, pBABE-hygro together with pCL-Eco (encoding gag, pol and ecotropic envelope).

    Article Title: VPS37A directs ESCRT recruitment for phagophore closure
    Article Snippet: The following plasmids were obtained from Hartmut Land (University of Rochester Medical Center, Rochester, NY), Jay Morgenstern (Imperial Cancer Research Fund, London, UK), Bob Weinberg (Whitehead Institute for Biomedical Research, Cambridge, MA), Feng Zhang (Massachusetts Institute of Technology, Cambridge, MA), Brett Stringer (QIMR Berghofer Medical Research Institute, Brisbane, Australia), Christopher Vakoc (Cold Spring Harbor Laboratory, Cold Spring Harbor, NY), Noboru Mizushima (The University of Tokyo, Tokyo, Japan), Wesley Sundquist (University of Utah School of Medicine, Salt Lake City, UT), Konrad Buessow (Max Planck Institute for Molecular Genetics, Berlin, Germany), and Hemmo Meyer (University of Duisburg-Essen, Essen, Germany) through Addgene: pBabe-Hygro (#1765; ), lentiCas9-Blast (#52962; ), lentiCRISPRv2 (puro; #98290; Brett Stringer Lab), LRG (Lenti_sgRNA_EFS_GFP, #65656; ), pMXs-IP-EGFP-mATG5 (#38196; ), pMXs-IP-EGFP-ULK1 (#38193; ), pMXs-IP-EGFP-LC3 (#38195; ), pEGFP-VPS4-E228Q (#80351; ), pQTEV-VPS28 (#34803; ), and pmCherry-GAL3 (#85662; ).

    Article Title: Mouse genetics suggests cell-context dependency for Myc-regulated metabolic enzymes during tumorigenesis.
    Article Snippet: For retroviruses the following plasmids from Addgene were used: pBABEHrasG12V-puro, pBABE-HrasG12V-hygro, MSCV-Myc-IRESGFP, pWZL-Myc-blasticidine, pBABE-puro, pBABE-hygro together with pCL-Eco (encoding gag, pol and ecotropic envelope).

    Article Title: Progerin reduces LAP2α-telomere association in Hutchinson-Gilford progeria
    Article Snippet: LAP2α was amplified from pTD15 (gift from Dr Roland Foisner, Vienna). cDNAs were cloned into retroviral vector pBABE-hygro (Addgene, Cambridge, MA) or doxycyclin-inducible lentiviral vector pTRIPZ (Open Biosystem, Singapore).

    Article Title: A Role for p53 in the Adaptation to Glutamine Starvation through the Expression of SLC1A3
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Software and Algorithms GraphPad Prism 7 GraphPad software N/A FlowJo software v.10.3 FlowJo N/A CRISPR Design Tool Feng Zhang Lab (MIT) http://tools.genome-engineering.org CRISPOR Design Tool Tefor Infrastructure http://crispor.tefor.net/crispor.py IPA Qiagen https://www.qiagenbioinformatics.com/ products/ingenuity-pathway-analysis/ TopHat2 Kim et al., 2013 https://ccb.jhu.edu/software/tophat/ index.shtml HTSeq v.0.10.0 Simon Anders (EMBL Heidelberg) https://htseq.readthedocs.io/en/ release_0.10.0/ DESeq2 Love et al., 2014 https://bioconductor.org/packages/ release/bioc/html/DESeq2.html Cufflinks Trapnell et al., 2012 http://cole-trapnell-lab.github.io/ cufflinks/cuffdiff/ TraceFinder Version 4.1 Thermo Fisher Scientific OPTON-30626 Image Studio Lite Version 5.2.5 LI-COR https://www.licor.com/bio/products/ software/image_studio/

    Article Title: CD44 correlates with longevity and enhances basal ATF6 activity and ER stress resistance.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER pBabe-hygro Morgenstern and Land68 Addgene (#1765) pBabe-hygro-hTert Counter et al.69 Addgene (#1773) pBabe-hygro-mouse-CD44 This paper N/A pBabe-hygro-NMR-CD44 This paper N/A pBabe-hygro-mouse-LDLR This paper N/A pBabe-hygro-human-ATF6 (wild-type) This paper N/A pBabe-hygro-human-ATF6 (Y392C) This paper N/A pBabe-hygro-human-ATF6 (Y392C) This paper N/A pBabe-puro-human-CD44 Godar et al.70 Addgene (#19127) pBabe-puro-CD44-ectodomain-KDEL This paper N/A pLKO.1 Stewart et al.71 Addgene (#8453) pLKO.1-shLuc This paper N/A pLKO.1-shCD44-1 This paper N/A pLKO.1-shCD44-2 This paper N/A pDonor-tBFP-NLS-Neo Katoh et al.72 80767 peSpCas9(1.1)-23sgRNA Katoh et al.72 80768 Software and algorithms GraphPad Prism7 https://www.graphpad.com/ N/A ProteinPilot https://sciex.com/products/software/ proteinpilot-software 5.0.1 Progenesis QI https://www.nonlinear.com/progenesis/qi/ 4.2 FlowJo https://www.flowjo.com/ 10.7.1 Enrichr https://maayanlab.cloud/Enrichr/ N/A DESeq2 https://bioconductor.org 1.12.0 EdgeR https://bioconductor.org 3.32.0 limma https://bioconductor.org 3.46.0 genefilter https://bioconductor.org 1.72.0 TCC https://bioconductor.org 1.30.0 RSEM https://github.com/deweylab/RSEM 1.3.0 Trimmomatic http://www.usadellab.org/cms/? page = trimmomatic 0.38

    Article Title: CUX1 Enhances Pancreatic Cancer Formation by Synergizing with KRAS and Inducing MEK/ERK-Dependent Proliferation
    Article Snippet: The oncogenic KrasG12D was cloned from the pBabe-puro vector, a kind gift from Channing Der (Addgene plasmid # 58902, Watertown, MA, USA), into the pBabe-hygro, a gift from Hartmut Land, Jay Morgenstern and Bob Weinberg (Addgene plasmid # 1765, Watertown, MA, USA) using the BamHI and SalI restriction sites.

    Multiple Displacement Amplification:

    Article Title: Vimentin intermediate filaments and filamentous actin form unexpected interpenetrating networks that redefine the cell cortex
    Article Snippet: The PCR product is purified from an agarose gel and inserted into pBABE-hygro (pBABE-hygro is a gift from Hartmut Land and Jay P. Morgenstern, Imperial Cancer Research Fund, Lincoln's Inn Fields, London ( ) [Addgene plasmid no. 1765; http://n2t.net/addgene:1765 ; RRID Addgene_1765]) cut with BamHI and EcoRI using In-Fusion (Clontech).

    Article Title: Mouse Genetics Suggests Cell-Context Dependency for Myc-Regulated Metabolic Enzymes during Tumorigenesis
    Article Snippet: For retroviruses the following plasmids from Addgene were used: pBABE-HrasG12V-puro, pBABE-HrasG12V-hygro, MSCV- Myc -IRES-GFP, pWZL-Myc-blasticidine, pBABE-puro, pBABE-hygro together with pCL-Eco (encoding gag, pol and ecotropic envelope).

    Article Title: VPS37A directs ESCRT recruitment for phagophore closure
    Article Snippet: The following plasmids were obtained from Hartmut Land (University of Rochester Medical Center, Rochester, NY), Jay Morgenstern (Imperial Cancer Research Fund, London, UK), Bob Weinberg (Whitehead Institute for Biomedical Research, Cambridge, MA), Feng Zhang (Massachusetts Institute of Technology, Cambridge, MA), Brett Stringer (QIMR Berghofer Medical Research Institute, Brisbane, Australia), Christopher Vakoc (Cold Spring Harbor Laboratory, Cold Spring Harbor, NY), Noboru Mizushima (The University of Tokyo, Tokyo, Japan), Wesley Sundquist (University of Utah School of Medicine, Salt Lake City, UT), Konrad Buessow (Max Planck Institute for Molecular Genetics, Berlin, Germany), and Hemmo Meyer (University of Duisburg-Essen, Essen, Germany) through Addgene: pBabe-Hygro (#1765; ), lentiCas9-Blast (#52962; ), lentiCRISPRv2 (puro; #98290; Brett Stringer Lab), LRG (Lenti_sgRNA_EFS_GFP, #65656; ), pMXs-IP-EGFP-mATG5 (#38196; ), pMXs-IP-EGFP-ULK1 (#38193; ), pMXs-IP-EGFP-LC3 (#38195; ), pEGFP-VPS4-E228Q (#80351; ), pQTEV-VPS28 (#34803; ), and pmCherry-GAL3 (#85662; ).

    Article Title: Mouse genetics suggests cell-context dependency for Myc-regulated metabolic enzymes during tumorigenesis.
    Article Snippet: For retroviruses the following plasmids from Addgene were used: pBABEHrasG12V-puro, pBABE-HrasG12V-hygro, MSCV-Myc-IRESGFP, pWZL-Myc-blasticidine, pBABE-puro, pBABE-hygro together with pCL-Eco (encoding gag, pol and ecotropic envelope).

    Article Title: Progerin reduces LAP2α-telomere association in Hutchinson-Gilford progeria
    Article Snippet: LAP2α was amplified from pTD15 (gift from Dr Roland Foisner, Vienna). cDNAs were cloned into retroviral vector pBABE-hygro (Addgene, Cambridge, MA) or doxycyclin-inducible lentiviral vector pTRIPZ (Open Biosystem, Singapore).

    Article Title: A Role for p53 in the Adaptation to Glutamine Starvation through the Expression of SLC1A3
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Software and Algorithms GraphPad Prism 7 GraphPad software N/A FlowJo software v.10.3 FlowJo N/A CRISPR Design Tool Feng Zhang Lab (MIT) http://tools.genome-engineering.org CRISPOR Design Tool Tefor Infrastructure http://crispor.tefor.net/crispor.py IPA Qiagen https://www.qiagenbioinformatics.com/ products/ingenuity-pathway-analysis/ TopHat2 Kim et al., 2013 https://ccb.jhu.edu/software/tophat/ index.shtml HTSeq v.0.10.0 Simon Anders (EMBL Heidelberg) https://htseq.readthedocs.io/en/ release_0.10.0/ DESeq2 Love et al., 2014 https://bioconductor.org/packages/ release/bioc/html/DESeq2.html Cufflinks Trapnell et al., 2012 http://cole-trapnell-lab.github.io/ cufflinks/cuffdiff/ TraceFinder Version 4.1 Thermo Fisher Scientific OPTON-30626 Image Studio Lite Version 5.2.5 LI-COR https://www.licor.com/bio/products/ software/image_studio/

    Article Title: CD44 correlates with longevity and enhances basal ATF6 activity and ER stress resistance.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER pBabe-hygro Morgenstern and Land68 Addgene (#1765) pBabe-hygro-hTert Counter et al.69 Addgene (#1773) pBabe-hygro-mouse-CD44 This paper N/A pBabe-hygro-NMR-CD44 This paper N/A pBabe-hygro-mouse-LDLR This paper N/A pBabe-hygro-human-ATF6 (wild-type) This paper N/A pBabe-hygro-human-ATF6 (Y392C) This paper N/A pBabe-hygro-human-ATF6 (Y392C) This paper N/A pBabe-puro-human-CD44 Godar et al.70 Addgene (#19127) pBabe-puro-CD44-ectodomain-KDEL This paper N/A pLKO.1 Stewart et al.71 Addgene (#8453) pLKO.1-shLuc This paper N/A pLKO.1-shCD44-1 This paper N/A pLKO.1-shCD44-2 This paper N/A pDonor-tBFP-NLS-Neo Katoh et al.72 80767 peSpCas9(1.1)-23sgRNA Katoh et al.72 80768 Software and algorithms GraphPad Prism7 https://www.graphpad.com/ N/A ProteinPilot https://sciex.com/products/software/ proteinpilot-software 5.0.1 Progenesis QI https://www.nonlinear.com/progenesis/qi/ 4.2 FlowJo https://www.flowjo.com/ 10.7.1 Enrichr https://maayanlab.cloud/Enrichr/ N/A DESeq2 https://bioconductor.org 1.12.0 EdgeR https://bioconductor.org 3.32.0 limma https://bioconductor.org 3.46.0 genefilter https://bioconductor.org 1.72.0 TCC https://bioconductor.org 1.30.0 RSEM https://github.com/deweylab/RSEM 1.3.0 Trimmomatic http://www.usadellab.org/cms/? page = trimmomatic 0.38

    Article Title: CUX1 Enhances Pancreatic Cancer Formation by Synergizing with KRAS and Inducing MEK/ERK-Dependent Proliferation
    Article Snippet: The oncogenic KrasG12D was cloned from the pBabe-puro vector, a kind gift from Channing Der (Addgene plasmid # 58902, Watertown, MA, USA), into the pBabe-hygro, a gift from Hartmut Land, Jay Morgenstern and Bob Weinberg (Addgene plasmid # 1765, Watertown, MA, USA) using the BamHI and SalI restriction sites.

    Recombinant:

    Article Title: Vimentin intermediate filaments and filamentous actin form unexpected interpenetrating networks that redefine the cell cortex
    Article Snippet: The PCR product is purified from an agarose gel and inserted into pBABE-hygro (pBABE-hygro is a gift from Hartmut Land and Jay P. Morgenstern, Imperial Cancer Research Fund, Lincoln's Inn Fields, London ( ) [Addgene plasmid no. 1765; http://n2t.net/addgene:1765 ; RRID Addgene_1765]) cut with BamHI and EcoRI using In-Fusion (Clontech).

    Article Title: Mouse Genetics Suggests Cell-Context Dependency for Myc-Regulated Metabolic Enzymes during Tumorigenesis
    Article Snippet: For retroviruses the following plasmids from Addgene were used: pBABE-HrasG12V-puro, pBABE-HrasG12V-hygro, MSCV- Myc -IRES-GFP, pWZL-Myc-blasticidine, pBABE-puro, pBABE-hygro together with pCL-Eco (encoding gag, pol and ecotropic envelope).

    Article Title: VPS37A directs ESCRT recruitment for phagophore closure
    Article Snippet: The following plasmids were obtained from Hartmut Land (University of Rochester Medical Center, Rochester, NY), Jay Morgenstern (Imperial Cancer Research Fund, London, UK), Bob Weinberg (Whitehead Institute for Biomedical Research, Cambridge, MA), Feng Zhang (Massachusetts Institute of Technology, Cambridge, MA), Brett Stringer (QIMR Berghofer Medical Research Institute, Brisbane, Australia), Christopher Vakoc (Cold Spring Harbor Laboratory, Cold Spring Harbor, NY), Noboru Mizushima (The University of Tokyo, Tokyo, Japan), Wesley Sundquist (University of Utah School of Medicine, Salt Lake City, UT), Konrad Buessow (Max Planck Institute for Molecular Genetics, Berlin, Germany), and Hemmo Meyer (University of Duisburg-Essen, Essen, Germany) through Addgene: pBabe-Hygro (#1765; ), lentiCas9-Blast (#52962; ), lentiCRISPRv2 (puro; #98290; Brett Stringer Lab), LRG (Lenti_sgRNA_EFS_GFP, #65656; ), pMXs-IP-EGFP-mATG5 (#38196; ), pMXs-IP-EGFP-ULK1 (#38193; ), pMXs-IP-EGFP-LC3 (#38195; ), pEGFP-VPS4-E228Q (#80351; ), pQTEV-VPS28 (#34803; ), and pmCherry-GAL3 (#85662; ).

    Article Title: Mouse genetics suggests cell-context dependency for Myc-regulated metabolic enzymes during tumorigenesis.
    Article Snippet: For retroviruses the following plasmids from Addgene were used: pBABEHrasG12V-puro, pBABE-HrasG12V-hygro, MSCV-Myc-IRESGFP, pWZL-Myc-blasticidine, pBABE-puro, pBABE-hygro together with pCL-Eco (encoding gag, pol and ecotropic envelope).

    Article Title: Progerin reduces LAP2α-telomere association in Hutchinson-Gilford progeria
    Article Snippet: LAP2α was amplified from pTD15 (gift from Dr Roland Foisner, Vienna). cDNAs were cloned into retroviral vector pBABE-hygro (Addgene, Cambridge, MA) or doxycyclin-inducible lentiviral vector pTRIPZ (Open Biosystem, Singapore).

    Article Title: A Role for p53 in the Adaptation to Glutamine Starvation through the Expression of SLC1A3
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Software and Algorithms GraphPad Prism 7 GraphPad software N/A FlowJo software v.10.3 FlowJo N/A CRISPR Design Tool Feng Zhang Lab (MIT) http://tools.genome-engineering.org CRISPOR Design Tool Tefor Infrastructure http://crispor.tefor.net/crispor.py IPA Qiagen https://www.qiagenbioinformatics.com/ products/ingenuity-pathway-analysis/ TopHat2 Kim et al., 2013 https://ccb.jhu.edu/software/tophat/ index.shtml HTSeq v.0.10.0 Simon Anders (EMBL Heidelberg) https://htseq.readthedocs.io/en/ release_0.10.0/ DESeq2 Love et al., 2014 https://bioconductor.org/packages/ release/bioc/html/DESeq2.html Cufflinks Trapnell et al., 2012 http://cole-trapnell-lab.github.io/ cufflinks/cuffdiff/ TraceFinder Version 4.1 Thermo Fisher Scientific OPTON-30626 Image Studio Lite Version 5.2.5 LI-COR https://www.licor.com/bio/products/ software/image_studio/

    Article Title: CD44 correlates with longevity and enhances basal ATF6 activity and ER stress resistance.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER pBabe-hygro Morgenstern and Land68 Addgene (#1765) pBabe-hygro-hTert Counter et al.69 Addgene (#1773) pBabe-hygro-mouse-CD44 This paper N/A pBabe-hygro-NMR-CD44 This paper N/A pBabe-hygro-mouse-LDLR This paper N/A pBabe-hygro-human-ATF6 (wild-type) This paper N/A pBabe-hygro-human-ATF6 (Y392C) This paper N/A pBabe-hygro-human-ATF6 (Y392C) This paper N/A pBabe-puro-human-CD44 Godar et al.70 Addgene (#19127) pBabe-puro-CD44-ectodomain-KDEL This paper N/A pLKO.1 Stewart et al.71 Addgene (#8453) pLKO.1-shLuc This paper N/A pLKO.1-shCD44-1 This paper N/A pLKO.1-shCD44-2 This paper N/A pDonor-tBFP-NLS-Neo Katoh et al.72 80767 peSpCas9(1.1)-23sgRNA Katoh et al.72 80768 Software and algorithms GraphPad Prism7 https://www.graphpad.com/ N/A ProteinPilot https://sciex.com/products/software/ proteinpilot-software 5.0.1 Progenesis QI https://www.nonlinear.com/progenesis/qi/ 4.2 FlowJo https://www.flowjo.com/ 10.7.1 Enrichr https://maayanlab.cloud/Enrichr/ N/A DESeq2 https://bioconductor.org 1.12.0 EdgeR https://bioconductor.org 3.32.0 limma https://bioconductor.org 3.46.0 genefilter https://bioconductor.org 1.72.0 TCC https://bioconductor.org 1.30.0 RSEM https://github.com/deweylab/RSEM 1.3.0 Trimmomatic http://www.usadellab.org/cms/? page = trimmomatic 0.38

    Article Title: CUX1 Enhances Pancreatic Cancer Formation by Synergizing with KRAS and Inducing MEK/ERK-Dependent Proliferation
    Article Snippet: The oncogenic KrasG12D was cloned from the pBabe-puro vector, a kind gift from Channing Der (Addgene plasmid # 58902, Watertown, MA, USA), into the pBabe-hygro, a gift from Hartmut Land, Jay Morgenstern and Bob Weinberg (Addgene plasmid # 1765, Watertown, MA, USA) using the BamHI and SalI restriction sites.

    Amplification:

    Article Title: Vimentin intermediate filaments and filamentous actin form unexpected interpenetrating networks that redefine the cell cortex
    Article Snippet: The PCR product is purified from an agarose gel and inserted into pBABE-hygro (pBABE-hygro is a gift from Hartmut Land and Jay P. Morgenstern, Imperial Cancer Research Fund, Lincoln's Inn Fields, London ( ) [Addgene plasmid no. 1765; http://n2t.net/addgene:1765 ; RRID Addgene_1765]) cut with BamHI and EcoRI using In-Fusion (Clontech).

    Article Title: Mouse Genetics Suggests Cell-Context Dependency for Myc-Regulated Metabolic Enzymes during Tumorigenesis
    Article Snippet: For retroviruses the following plasmids from Addgene were used: pBABE-HrasG12V-puro, pBABE-HrasG12V-hygro, MSCV- Myc -IRES-GFP, pWZL-Myc-blasticidine, pBABE-puro, pBABE-hygro together with pCL-Eco (encoding gag, pol and ecotropic envelope).

    Article Title: VPS37A directs ESCRT recruitment for phagophore closure
    Article Snippet: The following plasmids were obtained from Hartmut Land (University of Rochester Medical Center, Rochester, NY), Jay Morgenstern (Imperial Cancer Research Fund, London, UK), Bob Weinberg (Whitehead Institute for Biomedical Research, Cambridge, MA), Feng Zhang (Massachusetts Institute of Technology, Cambridge, MA), Brett Stringer (QIMR Berghofer Medical Research Institute, Brisbane, Australia), Christopher Vakoc (Cold Spring Harbor Laboratory, Cold Spring Harbor, NY), Noboru Mizushima (The University of Tokyo, Tokyo, Japan), Wesley Sundquist (University of Utah School of Medicine, Salt Lake City, UT), Konrad Buessow (Max Planck Institute for Molecular Genetics, Berlin, Germany), and Hemmo Meyer (University of Duisburg-Essen, Essen, Germany) through Addgene: pBabe-Hygro (#1765; ), lentiCas9-Blast (#52962; ), lentiCRISPRv2 (puro; #98290; Brett Stringer Lab), LRG (Lenti_sgRNA_EFS_GFP, #65656; ), pMXs-IP-EGFP-mATG5 (#38196; ), pMXs-IP-EGFP-ULK1 (#38193; ), pMXs-IP-EGFP-LC3 (#38195; ), pEGFP-VPS4-E228Q (#80351; ), pQTEV-VPS28 (#34803; ), and pmCherry-GAL3 (#85662; ).

    Article Title: Mouse genetics suggests cell-context dependency for Myc-regulated metabolic enzymes during tumorigenesis.
    Article Snippet: For retroviruses the following plasmids from Addgene were used: pBABEHrasG12V-puro, pBABE-HrasG12V-hygro, MSCV-Myc-IRESGFP, pWZL-Myc-blasticidine, pBABE-puro, pBABE-hygro together with pCL-Eco (encoding gag, pol and ecotropic envelope).

    Article Title: Progerin reduces LAP2α-telomere association in Hutchinson-Gilford progeria
    Article Snippet: LAP2α was amplified from pTD15 (gift from Dr Roland Foisner, Vienna). cDNAs were cloned into retroviral vector pBABE-hygro (Addgene, Cambridge, MA) or doxycyclin-inducible lentiviral vector pTRIPZ (Open Biosystem, Singapore).

    Article Title: A Role for p53 in the Adaptation to Glutamine Starvation through the Expression of SLC1A3
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Software and Algorithms GraphPad Prism 7 GraphPad software N/A FlowJo software v.10.3 FlowJo N/A CRISPR Design Tool Feng Zhang Lab (MIT) http://tools.genome-engineering.org CRISPOR Design Tool Tefor Infrastructure http://crispor.tefor.net/crispor.py IPA Qiagen https://www.qiagenbioinformatics.com/ products/ingenuity-pathway-analysis/ TopHat2 Kim et al., 2013 https://ccb.jhu.edu/software/tophat/ index.shtml HTSeq v.0.10.0 Simon Anders (EMBL Heidelberg) https://htseq.readthedocs.io/en/ release_0.10.0/ DESeq2 Love et al., 2014 https://bioconductor.org/packages/ release/bioc/html/DESeq2.html Cufflinks Trapnell et al., 2012 http://cole-trapnell-lab.github.io/ cufflinks/cuffdiff/ TraceFinder Version 4.1 Thermo Fisher Scientific OPTON-30626 Image Studio Lite Version 5.2.5 LI-COR https://www.licor.com/bio/products/ software/image_studio/

    Article Title: CD44 correlates with longevity and enhances basal ATF6 activity and ER stress resistance.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER pBabe-hygro Morgenstern and Land68 Addgene (#1765) pBabe-hygro-hTert Counter et al.69 Addgene (#1773) pBabe-hygro-mouse-CD44 This paper N/A pBabe-hygro-NMR-CD44 This paper N/A pBabe-hygro-mouse-LDLR This paper N/A pBabe-hygro-human-ATF6 (wild-type) This paper N/A pBabe-hygro-human-ATF6 (Y392C) This paper N/A pBabe-hygro-human-ATF6 (Y392C) This paper N/A pBabe-puro-human-CD44 Godar et al.70 Addgene (#19127) pBabe-puro-CD44-ectodomain-KDEL This paper N/A pLKO.1 Stewart et al.71 Addgene (#8453) pLKO.1-shLuc This paper N/A pLKO.1-shCD44-1 This paper N/A pLKO.1-shCD44-2 This paper N/A pDonor-tBFP-NLS-Neo Katoh et al.72 80767 peSpCas9(1.1)-23sgRNA Katoh et al.72 80768 Software and algorithms GraphPad Prism7 https://www.graphpad.com/ N/A ProteinPilot https://sciex.com/products/software/ proteinpilot-software 5.0.1 Progenesis QI https://www.nonlinear.com/progenesis/qi/ 4.2 FlowJo https://www.flowjo.com/ 10.7.1 Enrichr https://maayanlab.cloud/Enrichr/ N/A DESeq2 https://bioconductor.org 1.12.0 EdgeR https://bioconductor.org 3.32.0 limma https://bioconductor.org 3.46.0 genefilter https://bioconductor.org 1.72.0 TCC https://bioconductor.org 1.30.0 RSEM https://github.com/deweylab/RSEM 1.3.0 Trimmomatic http://www.usadellab.org/cms/? page = trimmomatic 0.38

    Article Title: CUX1 Enhances Pancreatic Cancer Formation by Synergizing with KRAS and Inducing MEK/ERK-Dependent Proliferation
    Article Snippet: The oncogenic KrasG12D was cloned from the pBabe-puro vector, a kind gift from Channing Der (Addgene plasmid # 58902, Watertown, MA, USA), into the pBabe-hygro, a gift from Hartmut Land, Jay Morgenstern and Bob Weinberg (Addgene plasmid # 1765, Watertown, MA, USA) using the BamHI and SalI restriction sites.



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    ( a,b ) HDR efficiencies with mammalian codon-optimized (+CO) and not codon-optimized (-CO) versions of TnpB and TnpB_CV2 in HEK293T cells at HDR reporter Target TB1 ( a ) and Target TB2 ( b ) ( n = 3 biological replicates). HDR was measured as percentage of EGFP-positive cells from BFP/mCherry double positive cells (BFP marks the guide-expressing construct; mCherry marks the nuclease-expressing construct). Each dot represents an individual biological replicate, and bars represent the mean ± standard deviation. (c) Western blot analysis of mammalian codon-optimized TnpB and TnpB_CV2 protein expression levels ( n = 3 biological replicates). Expression of each nuclease was performed together with ωRNA for Target TB1 in HEK293T cells. GAPDH was used as a loading control. ( d,e ) HDR efficiencies of mammalian codon-optimized TnpB and TnpB_CV2 combined with either Trim2 ωRNA scaffold or original ωRNA in HEK293T cells at HDR reporter Target TB1 ( d ) and Target TB2 ( e ). HDR was measured as percentage of EGFP-positive cells from BFP/mCherry double positive cells (BFP marks the guide-expressing construct; mCherry marks the nuclease-expressing construct) ( n = 2 biological replicates). Each dot represents an individual biological replicate, and bars represent the mean.

    Journal: bioRxiv

    Article Title: Directed evolution of compact RNA-guided nucleases for homology–directed repair in mammalian cells

    doi: 10.1101/2025.10.27.684765

    Figure Lengend Snippet: ( a,b ) HDR efficiencies with mammalian codon-optimized (+CO) and not codon-optimized (-CO) versions of TnpB and TnpB_CV2 in HEK293T cells at HDR reporter Target TB1 ( a ) and Target TB2 ( b ) ( n = 3 biological replicates). HDR was measured as percentage of EGFP-positive cells from BFP/mCherry double positive cells (BFP marks the guide-expressing construct; mCherry marks the nuclease-expressing construct). Each dot represents an individual biological replicate, and bars represent the mean ± standard deviation. (c) Western blot analysis of mammalian codon-optimized TnpB and TnpB_CV2 protein expression levels ( n = 3 biological replicates). Expression of each nuclease was performed together with ωRNA for Target TB1 in HEK293T cells. GAPDH was used as a loading control. ( d,e ) HDR efficiencies of mammalian codon-optimized TnpB and TnpB_CV2 combined with either Trim2 ωRNA scaffold or original ωRNA in HEK293T cells at HDR reporter Target TB1 ( d ) and Target TB2 ( e ). HDR was measured as percentage of EGFP-positive cells from BFP/mCherry double positive cells (BFP marks the guide-expressing construct; mCherry marks the nuclease-expressing construct) ( n = 2 biological replicates). Each dot represents an individual biological replicate, and bars represent the mean.

    Article Snippet: “All-in-one” construct for transient co-expression of TnpB and Trim2 ωRNA (also called ωRNA*) was obtained from Addgene (ID 212967).

    Techniques: Expressing, Construct, Standard Deviation, Western Blot, Control